Effect of polycaprolactone nanocapsules loaded with essential oils on biofilm formation by Staphylococcus aureus strains isolated from bovine mastitis cases

Bovine infectious mastitis is largely resistant to antibacterial treatment, mainly due mechanisms of bacterial resistance in the biofilms formed by Staphylococcus aureus. Melaleuca (MEO) and citronella essential oils (CEO) are promising agents for reducing or eliminating biofilms. Free melaleuca oil presented a medium Minimum Inhibitory Concentration (MIC) 0.625% Bactericidal (MBC) 1.250%, while free showed MIC MBC 0.313%. Thus, CEO MEO demonstrate bacteriostatic bactericidal potential. We generated polymeric nanocapsules containing evaluated their efficacy at S. Glass polypropylene spheres were used as test surfaces. To compare responses encapsulated oils, strains submitted 10 different procedures, using nanoencapsulated (EOs) vitro. observed no biofilm reduction MEO, nanoencapsulated. However, reduced formation on glass (p=0.03) tendency diminish (p=0.051). Despite vitro, formulation could be improved modify component polarity and, including obtain more interactions with surfaces matrix.